Lights, Camera, Action! Systematic variation in 2-D difference gel electrophoresis images
نویسندگان
چکیده
منابع مشابه
Informatics and Statistics for Analyzing 2-D Gel Electrophoresis Images
Despite recent progress in "shotgun" peptide separation by integrated liquid chromatography and mass spectrometry (LC/MS), proteome coverage and reproducibility are still limited with this approach and obtaining enough replicate runs for biomarker discovery is a challenge. For these reasons, recent research demonstrates that there is a continuing need for protein separation by two-dimensional g...
متن کاملFluorescent difference gel electrophoresis.
INTRODUCTION A bottleneck for high-throughput proteomic studies is image analysis. In conventional two-dimensional (2D) methodology, protein samples are separated on individual gels, stained, and quantified, followed by image comparison with computer-aided image analysis programs. Since different images are not always perfectly superimposable, image analysis is very time consuming. Fluorescent ...
متن کاملLaue crystallography: Lights! Camera! Action!
Recent advances in the Laue method of X-ray data collection from protein crystals have allowed very short-lived reaction intermediates to be observed successfully.
متن کاملIdentification of Differentially Expressed Proteins from Prostate Cancer Cell Lines by Fluorescence 2-D Difference Gel Electrophoresis (2-D DIGE)
Prostate cancer (PCa) is the most commonly diagnosed malignancy and the second most common largest cancer in males worldwide [1]. Approximately 2,30,000 patients were diagnosed prostate cancer in the US only in 2012. Nearly 40 000 persons were kill by the disease and its complications in the US annually which makes it the second leading cause of cancer death [2,3]. Prostate specific antigen (PS...
متن کاملAn Analysis System of 2-d Gel Electrophoresis Images for Genomic Scanning -dnainsight
RLGS(restriction landmark genomic scanning) method developed by Hayashizaki et al.[1] is one of the most powerful experimental procedures which can be applied to high-speed genome mapping. Through the method, restriction enzyme sites on genomic DNA are used as landmarks, being guideposts on the genome. These landmark information can be scanned over the whole genome by employing direct end-label...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: ELECTROPHORESIS
سال: 2007
ISSN: 0173-0835,1522-2683
DOI: 10.1002/elps.200600793